Composite

Part:BBa_K1604025:Design

Designed by: Turcato Alessandro   Group: iGEM15_UNITN-Trento   (2015-09-12)

araC-pBAD + proteorhodopsin + j23100 + blh + araC-pBAD + β-Carotene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2019
    Illegal NheI site found at 2042
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1740
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 4051
    Illegal BamHI site found at 6092
    Illegal XhoI site found at 1313
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2257
    Illegal NgoMIV site found at 5628
    Illegal NgoMIV site found at 5758
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1502
    Illegal AgeI site found at 1877
    Illegal AgeI site found at 3886
    Illegal AgeI site found at 4843
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI site found at 3868


Design Notes

Standard assembly between BBa_K1604022 and a PCR amplification of BBa_K1604010.

NOTE This device was built using a part from the Cambridge 2009 team. Be careful when operating this part, because the SpEI site in the suffix is missing. The part from Cambridge 2009 had this mistake and so the error was introduced in our part. We realized that after sequencing. Other than that the device work well.

Source

Arabinose inducible araC-pBAD promoter come from E. coli genome. Beta-carotene gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) genome. Protheorodopsin come from HF10_19P19, uncutured bacteria of SAR86 group, gammaprotoeobacteria.


  1. Walter, Jessica M., Derek Greenfield, Carlos Bustamante, and Jan Liphardt. "Light-powering Escherichia Coli with Proteorhodopsin." Proceedings of the National Academy of Sciences 104 (2007): 2408-2412.

  2. Martinez, A., A. S. Bradley, J. R. Waldbauer, R. E. Summons, and E. F. Delong. "Proteorhodopsin Photosystem Gene Expression Enables Photophosphorylation in a Heterologous Host" Proceedings of the National Academy of Sciences 104.13 (2007): 5590-595. Web.